Single-base mapping of m 6 A by an antibody-independent method

Key Points

• This research aims to develop a new method for mapping m6A modifications at a single-base level in the transcriptome.
• Introduced a novel antibody-independent technique for m6A mapping.
• Conducted a transcriptome-wide analysis to assess m6A site conservation.
• Generated reliable m6A maps demonstrating high precision at a single-base resolution.
• Revealed conserved m6A site patterns across different mammalian species.