Immunization with RotaTeq induced broader neutralizing antibody responses than ROTARIX, and inclusion of a single dose of RotaTeq enhanced G-type neutralization breadth.
Does RotaTeq induce broader neutralizing antibody responses compared to ROTARIX in vaccinated infants?
RotaTeq induces broader neutralizing antibody responses than ROTARIX, suggesting VP7 contributes to the breadth of vaccine-elicited immunity.
BACKGROUND: Rotavirus is a leading cause of pediatric diarrheal mortality. The rotavirus outer capsid consists of VP7 and VP4 proteins, which, respectively, determine viral G and P type and are primary targets of neutralizing antibodies. METHODS: To elucidate VP7-specific neutralizing antibody responses, we engineered monoreassortant rotaviruses each containing a human VP7 segment from a sequenced clinical specimen or a vaccine strain in an identical genetic background. We quantified replication and neutralization of engineered viruses using sera from infants vaccinated with monovalent ROTARIX or multivalent RotaTeq vaccines. RESULTS: Immunization with RotaTeq induced broader neutralizing antibody responses than ROTARIX. Inclusion of a single dose of RotaTeq in the schedule enhanced G-type neutralization breadth of vaccinated infant sera. Cell type-specific differences in infectivity, replication, and neutralization were detected for some monoreassortant viruses. CONCLUSIONS: These findings suggest that rotavirus VP7, independent of VP4, can contribute to cell tropism and the breadth of vaccine-elicited neutralizing antibody responses.
Diller et al. (Thu,) conducted a other in Rotavirus. RotaTeq vaccine vs. ROTARIX vaccine was evaluated on Neutralizing antibody responses and G-type neutralization breadth. Immunization with RotaTeq induced broader neutralizing antibody responses than ROTARIX, and inclusion of a single dose of RotaTeq enhanced G-type neutralization breadth.