A 1-hour bout of cycling exercise in healthy humans increased the circulation of over 300 EV-contained proteins, identifying a new paradigm for systemic tissue crosstalk.
Does a 1-hr bout of cycling exercise increase the secretion of EV-contained proteins in healthy humans?
Exercise induces the release of extracellular vesicles containing hundreds of proteins that can localize to the liver, representing a novel mechanism for systemic tissue crosstalk.
Exercise stimulates the release of molecules into the circulation, supporting the concept that inter-tissue signaling proteins are important mediators of adaptations to exercise. Recognizing that many circulating proteins are packaged in extracellular vesicles (EVs), we employed quantitative proteomic techniques to characterize the exercise-induced secretion of EV-contained proteins. Following a 1-hr bout of cycling exercise in healthy humans, we observed an increase in the circulation of over 300 proteins, with a notable enrichment of several classes of proteins that compose exosomes and small vesicles. Pulse-chase and intravital imaging experiments suggested EVs liberated by exercise have a propensity to localize in the liver and can transfer their protein cargo. Moreover, by employing arteriovenous balance studies across the contracting human limb, we identified several novel candidate myokines, released into circulation independently of classical secretion. These data identify a new paradigm by which tissue crosstalk during exercise can exert systemic biological effects.
Whitham et al. (Mon,) conducted a other in Healthy. Cycling exercise was evaluated on Exercise-induced secretion of EV-contained proteins. A 1-hour bout of cycling exercise in healthy humans increased the circulation of over 300 EV-contained proteins, identifying a new paradigm for systemic tissue crosstalk.
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