Abstract Cryopreservation is a widely used method for long-time sperm preservation, supporting the success of artificial insemination program and enhancing livestock genetics. Supplementation of extenders with egg yolk is a common practice for protection of sperm againt harmful effects of cryopreservation. This study aimed to evaluate the effects of different poultry egg yolk types (chicken, duck, and quail) at various concentrations (0, 5, 10, 15, and 20%) on the post-thaw quality of Boer goat sperm. Semen samples collected from four mature Boer male goats at weekly intervals were used. Semen samples were diluted in a Tris-based glycerol extender supplemented with five concentrations of three egg yolk types. The diluted semen was equilibrated at 15°C for 30 minutes, cooled to 5°C for 60 minutes, then exposed to liquid nitrogen vapor for 15 minutes before immersion and storage in liquid nitrogen. After 72 hours, semen was thawed at 37°C for 60 seconds, and sperm quality parameters were assessed. Results indicated that the 15% chicken egg yolk supplementation provided the best sperm preservation (P < 0.05), achieving the highest overall motility (66.69%), progressive motility (54.13%), viability (70.69%), membrane integrity (50.24%), acrosome integrity (80.12%) and the lowest DNA fragmentation rate (13.16%). These findings suggest that 15% chicken egg yolk in a Tris-glycerol extender optimally supports Boer goat sperm preservation. The results offer practical implications for improving artificial insemination success and establishing semen cryobanks for genetic conservation in tropical goat farming systems, particularly in regions such as the Mekong Delta, Vietnam.
Khương et al. (Tue,) studied this question.