Pharmacognosy Research,2025,17,4,1308-1314.DOI:10.5530/pres.20252328Published:August 2025Type:Original ArticleAuthors:Adavesh Holeyache, and Vishal Chaudhary Author(s) affiliations:Adavesh Holeyache*,#, Vishal Chaudhary# Department of Dravyaguna KAHER's, Shri BMK Ayurveda Mahavidyalaya Shahapur, Belagavi, Karnataka, INDIA. Abstract:Background: Phytochemicals from medicinal plants significantly impact healthcare. Piperine, a bioactive compound in Piper longum, exhibits diverse pharmacological effects, including analgesic, antipyretic, antioxidant, immune-stimulant, hepatoprotective, and bioavailability-enhancing properties. Traditionally, Piperine is extracted from both fruits (Pippali) and roots (Pippalimula) of Piper longum. Objectives: To quantify the Piperine content in Piper longum roots cultivated using bio formulations-Kunapa Jala and Vermiwash-on Piperine yield. Materials and Methods: Piper longum was cultivated with Random Block Design with two treatment groups (Kunapa Jala and Vermiwash), each with 12 replications. Roots were harvested, cleaned, dried, and powdered. High-Performance Thin-Layer Chromatography (HPTLC) was used to detect and quantify of Piperine. Results: HPTLC analysis revealed the presence of Piperine in both groups. Quantitative comparison showed a higher Piperine concentration in the Kunapa Jala treated group than in the Vermiwash group. Conclusion: Kunapa Jala enhances Piperine content in Piper longum roots in comparison to Vermiwash. This supports the potential of Vrukshayurveda and traditional formulations in improving the phytochemical yield of Pippali. Keywords:HPTLC analysis, Kunapa Jala, Piper longum, Piperine, Vermiwash, VrukshaayurvedaView:PDF (1.03 MB)
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