Two bacterial strains, designated E22-M8 T and B22-R8 T , were isolated from water sample collected from LungmuCo lake in Xizang of China. The 16S rRNA gene analysis revealed that strain E22-M8 T was most closely related to members of the genus Pseudidiomarina , exhibiting the highest sequence similarity of 97.38 and 97.39% to Pseudidiomarina halophila BH195 T and Pseudidiomarina terrestris 1APP75-27a T , respectively. While strain B22-R8 T showed the highest similarity to species of the genera Terrihabitans and Flaviflagellibacter : 97.69, 96.80 and 96.66% to ‘ Terrihabitans rhizophilus ’ PJ23 T , Terrihabitans soli IZ6 T and Flaviflagellibacter deserti SYSU D60017 T . For strain E22-M8 T , the digital DNA–DNA hybridization (dDDH) values with its closest relatives P. halophila BH195 T and P. terrestris 1APP75-27a T were 25.7 and 20.1%, respectively; the average nucleotide identity (ANI) values were 83.5 and 78%; and the average amino acid identity (AAI) values were 90.4 and 86.4%, all below the recognized species delineation thresholds. For strain B22-R8 T , the corresponding dDDH, ANI and AAI values with its closest relatives ‘ T. rhizophilus ’ PJ23 T , T. soli IZ6 T and F. deserti SYSU D60017 T also fell below the respective species thresholds. Biochemically and physiologically, both strains exhibited distinct traits that further supported their novelty. Based on comprehensive polyphasic analyses, strain E22-M8 T (=CGMCC 1.19205 T =KCTC 92346 T ) is proposed as a novel species named Pseudidiomarina xizangensis sp. nov., and strain B22-R8 T (=CGMCC 1.19187 T =KCTC 92343 T ) as a novel species designated Terrihabitans aquatilis sp. nov.
Luo et al. (Wed,) studied this question.