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February 27, 2026

Development and Validation of a Liquid Chromatograph-Tandem Mass Spectrometry Method for Quantifying Ganciclovir in Dried Blood Spots for Therapeutic Drug Monitoring.

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Authors

NTNaoki TamuraKIKotaro ItoharaSKSuguru Kouyama

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Overview

Demonstrates a new method for measuring ganciclovir in dried blood spots, suggesting improved therapeutic monitoring for CMV infection management.

Key Points

  • The study aimed to create a minimally invasive method for quantifying ganciclovir using dried blood spots and LC-MS/MS.
  • Developed a liquid chromatography-tandem mass spectrometry method for ganciclovir quantification.
  • Used dried blood spots for sample collection to reduce invasiveness.
  • Validated the method for accuracy, precision, and stability under refrigeration.
  • Demonstrated good linearity of 0.25-16 μg/mL for ganciclovir quantification.
  • Achieved reliable measurements within a hematocrit range of 30%-50%.
  • Identified methanol as the optimal extraction solvent for sample preparation.

Cite This Study

Tamura et al. (2026) studied this question.

synapsesocial.com/papers/69a1351ded1d949a99abeb0ehttps://doi.org/10.1002/bmc.70411
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Assessment of Dried Serum Spots (DSS) and Volumetric-Absorptive Microsampling (VAMS) Techniques in Therapeutic Drug Monitoring of (Val)Ganciclovir—Comparative Study in Analytical and Clinical Practice2024 · 7 citations
  2. 2Advances in Spectrophotometric and Oxidative Techniques for Medicine Quantification: A Review2026
  3. 3An enzymatic assay for rapid and minimally instrumented ganciclovir triphosphate monitoring in infants with congenital cytomegalovirus2025
  4. 4Development and Validation of an HPLC-UV Method for the Quantification of Acyclovir and Ganciclovir in the Plasma of Pediatric Immunocompromised Patients2024 · 6 citations
  5. 5Intracellular Ganciclovir Tri-Phosphate Concentrations in Children with Congenital Cytomegalovirus Infection2025 · 3 citations