A multicolour flow cytometry panel to characterize human amniotic fluid stem cells

Key Points

• The aim is to establish and optimize a multicolour flow cytometry panel for identifying human amniotic fluid stem cells.
• Utilized a 16 colour flow cytometry panel for characterization.
• Optimized titration and fixation/permeabilization for each antibody.
• Included multiple markers such as CD29, CD34, and OCT3/4.
• Successfully established a functional multicolour panel.
• Demonstrated effective characterization of stem cell markers.
• Enhanced identification of amniotic fluid stem cells using the optimized panel.