Key result
Quiescent adult rat ventricular myocytes cultured in defined medium undergo extensive phenotypic adaptation within 72 h, including a 30-50% decline in contractile function.
Population
Primary short-term cultures of adult rat ventricular myocytes
Comparison
Culture in defined medium for up to 72 hours vs Freshly isolated cells
Design
Preclinical
Follow-up
Up to 8 days
Authors
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May limit cultured adult rat myocyte use in functional studies; leaves open whether optimized conditions preserve native phenotype.
Quiescent adult rat ventricular myocytes in defined medium undergo extensive phenotypic adaptation within 72 hours, which may limit their suitability for electrophysiological and contractile function studies.
Ellingsen et al. (1993) studied Adult rat ventricular myocytes. Culturing in defined medium vs. Freshly isolated cells was evaluated on Phenotype and electromechanical function. Quiescent adult rat ventricular myocytes cultured in defined medium undergo extensive phenotypic adaptation within 72 h, including a 30-50% decline in contractile function.
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