Genetic Profiling of Primary Versus Metastatic Ewing Sarcoma for Therapeutic Target Identification

Figures

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  Figure 1. Volcano plot of differential expression of metastatic ES genes compared to primary ES genes. Green circles illustrate upregulated genes, red circles illustrate downregulated genes, and gray circles illustrate genes that did not meet one or both of the required statistical thresholds. Figures generated via QIAGEN Ingenuity Pathway Analysis (IPA) with permission from QIAGEN Silicon Valley.

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  Figure 2. Top 10 canonical pathways associated with metastatic ES versus primary ES. Green bars represent upregulated genes, and red bars represent downregulated genes. Figures generated via QIAGEN Ingenuity Pathway Analysis (IPA) with permission from QIAGEN Silicon Valley.

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  Figure 3. (A) Upstream regulator beta-estradiol. Predicted upregulation of beta-estradiol, as described in the Discussion; (B) Upstream regulator TGFB1. Predicted downregulation of TGFB1 contributes to the observed increased expression of gene candidates SFTPC and SFTPB. Figures generated via QIAGEN Ingenuity Pathway Analysis (IPA) with permission from QIAGEN Silicon Valley. The figures demonstrate the predicted downstream alterations due to variation in the expression of the upstream regulator in metastatic versus primary ES. Orange shape is predicted activation, blue shape is predicted inhibition, green shape is increased expression, red shape is decreased expression. Solid line and arrow is direct effect and dashed line and arrow is indirect effect. Orange line is leads to activation, blue line is leads to inhibition, and grey line is effect is not predicted.

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  Figure 4. (A) Top causal network JBI-802; (B) most activated causal network 3,4,5,3′,4′-pentachlorobiphenyl; (C) most inhibited causal network artemether. Figures generated via QIAGEN Ingenuity Pathway Analysis (IPA) with permission from QIAGEN Silicon Valley. The figures demonstrate the predicted downstream alterations due to variation in the expression of the upstream regulator in metastatic versus primary ES. Orange shape is predicted activation, blue shape is predicted inhibition, green shape is increased expression, red shape is decreased expression. Solid line and arrow is direct effect and dashed line and arrow is indirect effect. Orange line is leads to activation, blue line is leads to inhibition, and grey line is effect is not predicted.