AIMS: isolated from chronic wounds. MATERIALS AND METHODS: Growth in the presence and absence of CFS was evaluated, and biofilm formation was measured through bacterial growth on 96-well plates, followed by staining with safranin. Biofilm-associated genes were analyzed by qPCR, and a molecular fingerprinting of the CFS was performed. RESULTS: genes could not. Molecules different from the control were detected by HPLC-MS, which were not affected by heat or proteinase K. CONCLUSIONS: , and future studies may help to identify these molecules and broaden the comprehension of their mechanism of action.
Valente et al. (Tue,) studied this question.