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The application of a sulphatase andglucuronidase extract of molluscan origin to the hydrolysis of urinary steroid conjugates has been described.2. The effect of varying conditions of pH, time and enzyme concentration on the rate of hydrolysis of the conjugates has been studied.3. The influence of some urinary inorganic inhibitors on the rate of enzymic hydrolysis has been indicated.4. A comparison of the amount of neutral 17oxosteroids released by the methods of enzymic and acid hydrolysis has been made.5. Partition chromatography of urinary neutral 17-oxosteroids after both enzymic and acid hydro- lysis has been carried out.A difference in the distribution of various chromatographic fractions dependin"g on the method of hydrolysis was noted.
Blumberg et al. (Wed,) studied this question.