Mapping of yeast cytochrome c oxidase by fluorescence resonance energy transfer. Distances between subunit II, heme a, and cytochrome c bound to subunit III.

c bound to Subunit III + heme a, 25 A. Electron transfer from the heme group of ferrocytochrome c to the heme a groups of cytochrome c oxidase must thus bridge a considerable distance.The quantitative information obtained in this study should contribute to an understanding of how cytochrome c oxidase functions and how its components are arranged within the mitochondrial inner membrane.Cytochrome c oxidase is rapidly becoming one of the most intensely studied membrane proteins.The enzyme has been