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Prothrombin activation either by activated Factor X alone or by activated Factor X, Factor V, Phospholipid, and calcium ions results in the formation of the protease thrombin and, when thrombin proteolysis is prevented with diisopropyl fluorophosphate (iPrzP-F), a single activation fragment (Fragment 1.2) (ESMON,
Esmon et al. (Sun,) studied this question.
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