Crude initiation factor preparations from poliovirus-infected cells stimulated translation of poliovirus RNA but were inactive for host cell or vesicular stomatitis virus mRNAs.
Poliovirus infection specifically alters initiation factors to inhibit host and other viral mRNA translation while supporting its own RNA translation.
Crude initiation factor preparations from poliovirus-infected cells stimulated the translation of poliovirus RNA in vitro, but were inactive for the translation of host cell or vesicular stomatitis virus mRNA's. In contrast, similar preparations from either uninfected or vesicular stomatitis virus-infected cells supported the initiation of translation of host cell mRNA's and both viral mRNA's. These results reflect a specific alteration of some components(s) of the initiation factor preparation from poliovirus-infected cells which is consistent with the ability of the virus to inhibit the translation of host cell and vesicular stomatitis virus-directed protein synthesis.
Brown et al. (1980) studied Poliovirus infection (in vitro). Crude initiation factor preparations from poliovirus-infected cells vs. Preparations from uninfected or vesicular stomatitis virus-infected cells was evaluated on Translation of viral and host cell mRNAs in vitro. Crude initiation factor preparations from poliovirus-infected cells stimulated translation of poliovirus RNA but were inactive for host cell or vesicular stomatitis virus mRNAs.