April 1, 2001Open Access

Genetic Transformation of Yeast

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Abstract

Genetic transformation was first described by Griffith in 1928 and has since been demonstrated in a variety of organisms, including many species of fungi. This review focuses on the history and technology of the transformation of Saccharomyces cerevisiae. The application of protocols developed for S. cerevisiae to other important yeast species is discussed. The protocols for transformation by spheroplasting, LiAc/ssDNA/PEG, and electroporation are compared, and possible mechanisms for transformation are discussed.

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Authors

R. Daniel Gietz

University of Manitoba

Robin A. Woods

University of Winnipeg

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BioTechniques

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University of Manitoba

University of Winnipeg

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Gietz et al. (Sun,) studied this question.

synapsesocial.com/papers/6a10518f8090e499da60f842 — DOI: https://doi.org/10.2144/01304rv02

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Fast Kinetic-Studies of Plasmid DNA Transfer in Intact Yeast Cells Mediated by Electropulsation· 1995 · 27 citations
The Significance of Pneumococcal Types· 1928 · 1,088 citations
[56] Molecular genetic analysis of fission yeast Schizosaccharomyces pombe· 1991 · 3,791 citations
Studies on the Chemical Nature of the Substance Inducing Transformation of Pneumococcal Types· 2017 · 756 citations
Development of a transformation system for the yeast Yamadazyma (Pichia) ohmeri· 1994 · 1 citations

Also consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

Fast Kinetic-Studies of Plasmid DNA Transfer in Intact Yeast Cells Mediated by Electropulsation· 1995 · 27 citations
The Significance of Pneumococcal Types· 1928 · 1,088 citations
[56] Molecular genetic analysis of fission yeast Schizosaccharomyces pombe· 1991 · 3,791 citations
Studies on the Chemical Nature of the Substance Inducing Transformation of Pneumococcal Types· 2017 · 756 citations
Development of a transformation system for the yeast Yamadazyma (Pichia) ohmeri· 1994 · 1 citations

Genetic Transformation of Yeast

Key Points

Abstract

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Authors

Journals

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References and Citations

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Discussion

Cite this study

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