Key points are not available for this paper at this time.
Materials-Guanidinium chloride, cesium chloride, and toluidine blue were obtained from BDH Chemicals, Ltd.Phenylmethylsulfonyl fluoride, iodoacetic acid, papain, bacterial collagenase, and hyaluronic acid (from human umbilical cord) were from Sigma Chemical Co.Pepsin was from Worthington Biochemical Cow. Chondroitinase ABC and its disaccharide degradation products of chondroitin sulfate were from Miles Laboratories, Inc. Acrylamide, N,N'-methylenebisacrylamide, and Coomassie
Roughley et al. (Tue,) studied this question.