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Zymography is the electrophoretic separation of proteins through a polyacrylamide gel containing a proteolytic substrate. After denaturing (but nonreducing) electrophoresis, proteins are renatured and incubated in an appropriate buffer for proteolytic activity. Clear zones of lysis in the stained gel indicated active proteinases. Reverse zymography is a similar technique to detect proteinase inhibitors. After renaturing of proteins, the gel is incubated with metalloproteinases which digest the substrate incorporated into the gel. Inhibitors are shown as dark zones of inhibition against a clear background upon staining.
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Hawkes et al. (Fri,) studied this question.
www.synapsesocial.com/papers/69ea773e186fc979e9a81b2a — DOI: https://doi.org/10.1007/978-1-60327-299-5_16
Susan P. Hawkes
Hongxia Li
Gary T. Taniguchi
University of California, San Francisco
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