A 5-h PCR assay with colorimetric microwell detection for enteroviral meningitis demonstrated a sensitivity of 94.7%, specificity of 97.4%, PPV of 94.7%, and NPV of 97.4%.
Observational
A 5-h, user-friendly PCR assay for the diagnosis of enteroviral meningitis was developed. Reverse transcription and amplification were performed in a one-step reaction using rTth polymerase. Carryover contamination was prevented with dUTP and uracil N-glycosylate. Detection was performed colorimetrically on a microwell titer plate. Sensitivity, specificity, positive predictive value, and negative predictive value were 94.7, 97.4, 94.7, and 97.4%, respectively.
Rotbart et al. (Sat,) conducted a observational in Enteroviral meningitis. 5-h PCR assay with colorimetric microwell detection was evaluated on Diagnostic accuracy (sensitivity, specificity, positive predictive value, and negative predictive value). A 5-h PCR assay with colorimetric microwell detection for enteroviral meningitis demonstrated a sensitivity of 94.7%, specificity of 97.4%, PPV of 94.7%, and NPV of 97.4%.
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