Key result
A 5-h PCR assay with colorimetric microwell detection for enteroviral meningitis demonstrated a sensitivity of 94.7%, specificity of 97.4%, PPV of 94.7%, and NPV of 97.4%.
Why the study?
Does a 5-h PCR assay with colorimetric microwell detection accurately diagnose enteroviral meningitis?
Observational
Does a 5-h PCR assay with colorimetric microwell detection accurately diagnose enteroviral meningitis?
A 5-hour PCR assay with colorimetric microwell detection provides high sensitivity and specificity for diagnosing enteroviral meningitis.
May support rapid enteroviral meningitis diagnosis; leaves open larger prospective validation before routine use.
A 5-h, user-friendly PCR assay for the diagnosis of enteroviral meningitis was developed. Reverse transcription and amplification were performed in a one-step reaction using rTth polymerase. Carryover contamination was prevented with dUTP and uracil N-glycosylate. Detection was performed colorimetrically on a microwell titer plate. Sensitivity, specificity, positive predictive value, and negative predictive value were 94.7, 97.4, 94.7, and 97.4%, respectively.
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Rotbart et al. (1994) conducted an observational in Enteroviral meningitis. 5-h PCR assay with colorimetric microwell detection was evaluated on Diagnostic accuracy (sensitivity, specificity, positive predictive value, and negative predictive value). A 5-h PCR assay with colorimetric microwell detection for enteroviral meningitis demonstrated a sensitivity of 94.7%, specificity of 97.4%, PPV of 94.7%, and NPV of 97.4%.
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