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The aerobic oxidation of xanthine by rat liver supernatant was greatly stimulated by the addition of methylene blue or of NAD+: the latter was reduced during the reaction. Storage of the supernatant at -20 brought about an enhancement of the xanthine oxidation rate measured without addition of cofactors. A similar "activation" was caused by prior incubation at 37 of the unfractionated liver homogenate, or of the supernatant separated after sonic disruption of the homogenate. The same effect was obtained by treatment with solvents, or by prior incubation at 37 of the supernatant in the presence of proteolytic enzymes or under anaerobic conditions. The presence of xanthine accelerated the effect of proteolytic enzymes and of anaerobiosis. Only the changes caused by anaerobiosis could be reversed by incubating the supernatant in air before the assay.
Stirpe et al. (Tue,) studied this question.