Mapping of the Proteinase B Structural Gene Prb1, in Saccharomyces Cerevisiae and Identification of Nonsense Alleles Within the Locus

We report the mapping of the structural gene for proteinase B, PRB1. It is located 1.1 cM proximal to CAN1 on the left arm of chromosome V of Saccharomyces cerevisiae. We have identified 34 amber and 12 ochre mutations among the 126 prb1 mutations in our collection.