Gradient Gel Electrophoresis of Human Plasma Lipoproteins

Abstract A rapid and simple method for separating and identifying plasma lipoproteins is described. The technique utilizes the "molecular sieving" properties of continuous concave gradient electrophoresis on a slab of polyacrylamide (4 to 26%) gel. Gradient gel electrophoresis is particularly suitable for separating lipoproteins according to their molecular weight. The technique can be used to demonstrate the major lipoprotein classes in normal plasma and to identify abnormalities of lipoprotein metabolism. Components of a sample are better resolved by gradient gel electrophoresis than by electrophoresis on paper or cellulose acetate. The results obtained compare favorably with those achieved by preparative ultracentrifugation. The technique is easily and rapidly performed in a routine clinical laboratory.