Vagal stimulation produced localized hyperpolarization of cells in the N region of the AV node, correlating with strong acetylcholinesterase staining and identifying the midnodal region as the focus of vagal effect.
Vagal-induced depression of AV nodal conduction is localized to the midnodal (N) region, where acetylcholine release and effector cell hyperpolarization are concentrated.
The mechanisms responsible for slowing cardiac impulse conduction through the atrioventricular (AV) node are not well understood but include anatomical architecture, presence of cells with diverse electrophysiological characteristics, and modulation by autonomic nervous system. The present study was designed to determine the site of vagally induced slowing of conduction through the AV node. We attempted to correlate the electrophysiological response of AV nodal cells to postganglionic vagal stimulation applied in different regions of the node with the morphological findings and patterns of acetylcholinesterase-positive staining of nodal tissue. This multifaceted approach revealed that vagal stimulation produced localized hyperpolarization of the cells from the N region of the AV node, which correlated with the strong acetylcholinesterase positive staining of the central nodal area. In contrast, the density of the acetylcholinesterase staining decreased toward both the AN and His bundle regions, whereas vagal stimulation had a negligible effect on the cells from these regions. These results suggest that vagal-induced depression of AV nodal conduction is produced by release of acetylcholine predominantly around the midnodal region and the depressive action of acetylcholine is concentrated on the cells occupying the same region (i.e., the N cells). Thus, there appears to be a close juxtaposition of nerve elements and effector cells in the midnodal region of the AV node. This unique combination of available neuromediator and responding cells with hyperpolarization and depressed action potential determines the midnodal region as the focus of vagal effect on AV nodal conduction.
Imaizumi et al. (Sat,) conducted a other in Atrioventricular nodal conduction physiology. Vagal stimulation was evaluated on Electrophysiological response of AV nodal cells and acetylcholinesterase-positive staining. Vagal stimulation produced localized hyperpolarization of cells in the N region of the AV node, correlating with strong acetylcholinesterase staining and identifying the midnodal region as the focus of vagal effect.