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A survey of applications of high speed liquid chromatography to the analysis of steroids is presented. Sensitivities of ultraviolet and refractive index detectors are discussed and the use of a 2,4-dinitrophenylhydrazine derivative to bring the sensitivity of the UV detector down to the one nanogram level is reported. High speed separations by conventional liquid-liquid partition chromatography, reversed-phase partition chromatography and ion-exchange chromatography are shown for all major types of steroids, including estrogens, androgens, progestogens and adrenocortical hormones. In addition separations are shown for a biologically important insect hormone, ecdysone, and for several steroid conjugates such as digoxin/digitoxin and estradiol glucosiduronic acid.
Henry et al. (Wed,) studied this question.