Time-resolved Mass Spectrometry of Tyrosine Phosphorylation Sites in the Epidermal Growth Factor Receptor Signaling Network Reveals Dynamic Modules

Ligand binding to cell surface receptors initiates a cascade of signaling events regulated by dynamic phosphorylation events on a multitude of pathway proteins. Quantitative features, including intensity, timing, and duration of phosphorylation of particular residues, may play a role in determining cellular response, but experimental data required for analysis of these features have not previously been available. To understand dynamic of signaling have a of phosphorylation of on of in a of of to of and by and of phosphorylation to of phosphorylation on a of a of data and of phosphorylation and proteins. phosphorylation for data of phosphorylation by in of of phosphorylation dynamic in signaling particular cellular of and phosphorylation these of and of these proteins. analysis and of data in to of and Ligand binding to cell surface receptors initiates a cascade of signaling events regulated by dynamic phosphorylation events on a multitude of pathway proteins. Quantitative features, including intensity, timing, and duration of phosphorylation of particular residues, may play a role in determining cellular response, but experimental data required for analysis of these features have not previously been available. 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