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Airborne bacteria, the protease content of the airborne dust and the concentration of 2–4 μm particles in the cardroom air were all found to correlate significantly with the prevalence of byssinotic symptoms. Byssinotic workers with 20 years' or longer cotton spinning experience had a statistically significant excess of symptoms of chronic bronchitis and asthma compared with non-byssinotic workers matched for age, cardroom experience and smoking habits.
Cinkotai et al. (1978) studied this question.