Key points are not available for this paper at this time.
As a step toward propagation of synthetic genomes, we completely replaced the genome of a bacterial cell with one from another species by transplanting a whole genome as naked DNA. Intact genomic DNA from Mycoplasma mycoides large colony (LC), virtually free of protein, was transplanted into Mycoplasma capricolum cells by polyethylene glycol-mediated transformation. Cells selected for tetracycline resistance, carried by the M. mycoides LC chromosome, contain the complete donor genome and are free of detectable recipient genomic sequences. These cells that result from genome transplantation are phenotypically identical to the M. mycoides LC donor strain as judged by several criteria.
Building similarity graph...
Analyzing shared references across papers
Loading...
Carole Lartigue
John I. Glass
Nina Alperovich
Science
J. Craig Venter Institute
Building similarity graph...
Analyzing shared references across papers
Loading...
Lartigue et al. (Thu,) studied this question.
www.synapsesocial.com/papers/6a01cb0dc3b9b1dfa5cae30f — DOI: https://doi.org/10.1126/science.1144622