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Current methods to improve the engineering properties of sands are diverse with respect to methodology, treatment uniformity, cost, environmental impact, site accessibility requirements, etc. All of these methods have benefits and drawbacks, and there continues to be a need to explore new possibilities of soil improvement, particularly as suitable land for development becomes more scarce. This paper presents the results of a study in which natural microbial biological processes were used to engineer a cemented soil matrix within initially loose, collapsible sand. Microbially induced calcite precipitation (MICP) was achieved using the microorganism Bacillus pasteurii, an aerobic bacterium pervasive in natural soil deposits. The microbes were introduced to the sand specimens in a liquid growth medium amended with urea and a dissolved calcium source. Subsequent cementation treatments were passed through the specimen to increase the cementation level of the sand particle matrix. The results of both MICP- and gypsum-cemented specimens were assessed nondestructively by measuring the shear wave velocity with bender elements. A series of isotropically consolidated undrained compression (CIUC) triaxial tests indicate that the MICP-treated specimens exhibit a noncollapse strain softening shear behavior, with a higher initial shear stiffness and ultimate shear capacity than untreated loose specimens. This behavior is similar to that of the gypsum-cemented specimens, which represent typical cemented sand behavior. SEM microscopy verified formation of a cemented sand matrix with a concentration of precipitated calcite forming bonds at particle-particle contacts. X-ray compositional mapping confirmed that the observed cement bonds were comprised of calcite.
DeJong et al. (Wed,) studied this question.