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A synthetic conjugate, prepared by covalent binding of a lysozyme fragment (sequence 64-83, denoted "loop" peptide) to a synthetic branched polypeptide, elicited in rabbits the formation of antibodies with specificity directed against a unique region in native lysozyme. These anti-"loop" antibodies were isolated immunospecifically on a lysozyme-cellulose immunoadsorbent. Antibodies with a similar specificity were isolated from antilysozyme sera with an immunoadsorbent prepared from the same "loop" peptide. The capacity of the anti-"loop" antibodies to distinguish between the "loop" peptide, containing a disulfide bridge, and the open-chain peptide derived from it suggests that they are directed against a conformation-dependent determinant.
Arnon et al. (Wed,) studied this question.