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Mice deficient in 14-3-3ε exhibit abnormal neuronal migration and die perinatally. We report here the first large-scale analysis of 14-3-3 interacting partners from primary animal tissue, identifying from embryonic murine brain 163 14-3-3ε interacting proteins and 85 phosphorylation sites on these proteins. Phosphorylation of the deubiquitinating enzyme USP8 at serine 680 was found essential for its interaction with 14-3-3ε and for maintaining USP8 in the cytosol. Keywords: phosphoproteomics • 14-3-3 • epsilon • brain development • kinase • phosphorylation • mass spectrometry • deubiquitination
Ballif et al. (Wed,) studied this question.