Computed Tomography Monitoring of Radiation-Induced Lung Fibrosis in Mice

RATIONALE AND OBJECTIVES: To identify characteristics of lung fibrosis in a mouse model after radiotherapy (RT) using thin-section computed tomography (CT), histology and clinical parameters. MATERIALS AND METHOD: Using a multislice CT-scanner, follow-up chest CT scans of 10 out of 72 included mice (C57BL/6J, 36 control mice, 36 mice (20Gy)) were performed every 2 weeks until week 26 after RT. Hounsfield units (HU) and cardiothoracic ratio (CTR) were measured, and a multireader analysis on characteristic lung changes was performed and correlated with histology and clinical parameters. RESULTS: From weeks 4 to 8 after RT changes in histology (leukocyte count, extraalveolar edema, P 0.84). CONCLUSION: Thin-section CT in a mouse model is capable of detecting the development of lung fibrosis after RT prior to the onset of clinical deterioration.