Knockdown of LPL via shRNA or Orlistat in goat mammary epithelial cells altered the expression of fatty acid synthesis genes, suggesting LPL plays a crucial role in milk fat synthesis.
LPL plays a crucial role in fatty acid synthesis in goat mammary epithelial cells, with expression patterns correlating with milk fat percentage during lactation.
Lipoprotein lipase (LPL) serves as a central factor in hydrolysis of triacylglycerol and uptake of free fatty acids from the plasma. However, there are limited data concerning the action of LPL on the regulation of milk fat synthesis in goat mammary gland. In this investigation, we describe the cloning and sequencing of the LPL gene from Xinong Saanen dairy goat mammary gland, along with a study of its phylogenetic relationships. Sequence analysis showed that goat LPL shares similarities with other species including sheep, bovine, human and mouse. LPL mRNA expression in various tissues determined by RT-qPCR revealed the highest expression in white adipose tissue, with lower expression in heart, lung, spleen, rumen, small intestine, mammary gland, and kidney. Expression was almost undetectable in liver and muscle. The expression profiles of LPL gene in mammary gland at early, peak, mid, late lactation, and the dry period were also measured. Compared with the dry period, LPL mRNA expression was markedly greater at early lactation. However, compared with early lactation, the expression was lower at peak lactation and mid lactation. Despite those differences, LPL mRNA expression was still greater at peak, mid, and late lactation compared with the dry period. Using goat mammary epithelial cells (GMEC), the in vitro knockdown of LPL via shRNA or with Orlistat resulted in a similar degree of down-regulation of LPL (respectively). Furthermore, knockdown of LPL was associated with reduced mRNA expression of SREBF1, FASN, LIPE and PPARG but greater expression of FFAR3. There was no effect on ACACA expression. Orlistat decreased expression of LIPE, FASN, ACACA, and PPARG, and increased FFAR3 and SREBF1 expression. The pattern of LPL expression was similar to the changes in milk fat percentage in lactating goats. Taken together, results suggest that LPL may play a crucial role in fatty acid synthesis.
Zhao et al. (Tue,) conducted a other in Fatty acid synthesis in goat mammary epithelial cells. LPL knockdown via shRNA or Orlistat was evaluated on Expression of genes related to fatty acid synthesis (SREBF1, FASN, LIPE, PPARG, FFAR3, ACACA). Knockdown of LPL via shRNA or Orlistat in goat mammary epithelial cells altered the expression of fatty acid synthesis genes, suggesting LPL plays a crucial role in milk fat synthesis.