A transient outward current in isolated cells from the crista terminalis of rabbit heart.

Voltage-clamp experiments were carried out with the objective of identifying and characterizing the time- and voltage-dependent properties of a transient outward current recorded in single myocytes from the crista terminalis region of the rabbit heart. A collagenase enzymic dispersion procedure similar to that described by Desilets a transient outward current was consistently recorded following blockade of ICa with Cd2+ (5 X 10(-4) M). The time and voltage dependence of the activation and inactivation of this current were measured. Its steady-state inactivation curve spans the voltage range -70 to -10 mV, and it is activated between -20 and +10 mV. The reversal potential of this transient outward current is approximately -75 mV in K+O 5 mM, suggesting that it is carried mainly by K+. This transient outward current can be inhibited completely by external application of 4-aminopyridine (4-AP, 3 mM). The time- and voltage-dependent properties, the reversal potential, and the sensitivity to 4-AP of this transient outward current are all very similar to those of a transient outward current first identified in molluscan neurones. Hence, we have labelled it, IA. Selective inhibition of IA and knowledge of its voltage- and time-dependent properties yield specific predictions concerning its role in the action potential of isolated crista terminalis cells. Consistent with these predictions, a decrease in stimulus rate is found to decrease the duration of the action potential and vice versa; and application of effective doses of 4-AP results in a substantial lengthening of the action potential. These results are discussed in terms of the possible physiological role of IA in subsidiary or follower pace-maker tissue, and the anatomical and physiological heterogeneity of the sino-atrial node region of the rabbit heart.