High-fat diet-induced obesity in female mice significantly decreased endothelium-dependent relaxation of the aorta to acetylcholine (P < 0.05), which was corrected by SOD and sodium salicylate.
In a mouse model, high-fat diet-induced obesity impaired endothelium-dependent relaxation, which was corrected by antioxidant and anti-inflammatory treatments.
p-value: p=<0.05
OBJECTIVE: We investigated the effects of high-fat diet-induced obesity on vascular proinflammatory factors and oxidative stress on endothelium-dependent relaxation of the aorta. METHODS: Female Swiss mice were submitted to a high-fat diet for 16 weeks. At the end of the experimental period, we evaluated blood pressure, relaxation in response to acetylcholine in aortic rings in the absence and the presence of the superoxide anion scavenger, superoxide dismutase (SOD, 150 U/ml), and the nuclear factor (NF)-κB inhibitor, sodium salicylate (5 mmol/l). Aortic protein expression of endothelial nitric oxide synthase, Cu/Zn-SOD, NF-κB, IκB-α, and proinflammatory cytokines were also evaluated. RESULTS: Obese mice presented higher systolic and diastolic blood pressure than control mice (P < 0.05). The relaxation of aortas to acetylcholine, but not to sodium nitroprusside, was significantly decreased in obese mice and was corrected by both SOD and sodium salicylate (P < 0.05). The protein expression of endothelial nitric oxide synthase and Cu/Zn-SOD was significantly decreased in aorta from obese mice (P < 0.05). Total p65 NF-κB subunit protein expression was not affected by obesity, but the protein expression of NF-κB inhibitor IκB-α was lower in aorta from obese mice (P < 0.05). There were no significant differences in the interleukin (IL)-1β and IL-6 protein expression between groups. In contrast, the expression of TNF-α was significantly increased in aortas from obese mice. CONCLUSION: Our results suggest that the reduced antioxidant defense and the local NF-κB pathway play an important role in the impairment of endothelium-dependent relaxation in aorta from obese mice.
Kobayasi et al. (Thu,) conducted a other in High-fat diet-induced obesity. High-fat diet vs. Control mice was evaluated on Endothelium-dependent relaxation of the aorta in response to acetylcholine (p=<0.05). High-fat diet-induced obesity in female mice significantly decreased endothelium-dependent relaxation of the aorta to acetylcholine (P < 0.05), which was corrected by SOD and sodium salicylate.