The 3' terminal sequence of the minus strand of polio replicative form is an exact complement to the 5' end of polio RNA, suggesting the 5' terminal U is genetically coded.
The study elucidates the structure of the poliovirus replicative form, indicating that the 5' terminal U is genetically coded and identifying a protruding single-stranded poly(A) tail.
The structure of polio replicative form (RF) has been investigated by 3' end labeling and the use of polynucleotide phosphorylase to now allow a complete composite of the RF structure. The evidence presented indicates that the 3' terminal sequence of the minus strand is an exact complement to the 5' end of polio RNA. This suggests that the 5' terminal U of polio RNA is genetically coded. Other data is presented to show that in addition to the genetically coded poly(A) tract of the plus strand in RF, a single-stranded poly(A) tail protrudes beyond the double-stranded RNA.
Larsen et al. (1980) studied Poliovirus. 3' end labeling and polynucleotide phosphorylase was evaluated on Structure of polio replicative form. The 3' terminal sequence of the minus strand of polio replicative form is an exact complement to the 5' end of polio RNA, suggesting the 5' terminal U is genetically coded.