Transgenic expression of aberrant cardiac troponin T variants found in animals with dilated cardiomyopathy resulted in contractile changes in mouse cardiomyocytes.
Does aberrant splicing of cardiac troponin T cause contractile changes and contribute to dilated cardiomyopathy in animal models?
Aberrant splicing of cardiac troponin T alters its structure and function, leading to contractile changes that may contribute to the pathogenesis of dilated cardiomyopathy.
Adult cardiac muscle normally expresses a single cardiac troponin T (cTnT). As a potential pathogenic mechanism for turkey dilated cardiomyopathy, the splice-out of a normally constitutive exon generates an additional low molecular weight cTnT with altered conformation and function. We further found that aberrant splicing of cTnT also occurs in several mammals correlating to dilated cardiomyopathy. Skipping of the same exon as that in the turkey was found in the canine cTnT. Splice-out of the adjacent exon 6 occurred in the guinea pig cTnT. Retention of the embryonic exon 5 was found in the cTnT of cat, dog, and guinea pig. These aberrant splicing variants significantly altered the structure of cTnT to sustain functional effects as that in the myopathic turkey cTnT. The genomic sequence of canine cTnT gene shows no specific alterations. However, the alternative splicing patterns of canine cTnT are different in developing cardiac and skeletal muscles, suggesting abnormality of trans-regulatory factors. Transgenic expression of the aberrant cTnT variants resulted in contractile changes in mouse cardiomyocytes. The findings support the hypothesis that thin filament heterogeneity due to the co-expression of alternatively spliced cTnT variants may desynchronize myocardial contraction and contribute to the pathogenesis and pathophysiology of cardiomyopathy and heart failure.
Biesiadecki et al. (Sun,) conducted a other in Dilated cardiomyopathy. Transgenic expression of aberrant cTnT variants was evaluated on Contractile changes in mouse cardiomyocytes. Transgenic expression of aberrant cardiac troponin T variants found in animals with dilated cardiomyopathy resulted in contractile changes in mouse cardiomyocytes.