An improved colour reagent for the determination of blood glucose by the oxidase system

Key Points

• To develop a sensitive chromogenic reagent system using 4-aminophenazone and sulphonated 2,4-dichlorophenol for determining hydrogen peroxide generated during glucose oxidation.
• Coupled 4-aminophenazone with sulphonated 2,4-dichlorophenol to detect hydrogen peroxide produced by glucose oxidase.
• Quantified chromophore formation spectrophotometrically at 515 nm using 10-mm path length cells in a 4 ml reaction volume.
• Measurement of 20 µg glucose in a 4 ml volume generated an optical density of 0.61 at 515 nm.
• Achieved a high molecular absorption coefficient of 22,000 for the resulting colored product.