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The virulence of strains of the three types of poliovirus have been examined by the subcutaneous injection of monkeys. The strains used were the Sabin attenuated Types 1, 2 and 3, Cox attenuated Types 1, 2 and 3, the Brunenders Type 1 partially attenuated strain, MEF-1, Type 2 and Saukett, Type 3. The results obtained indicated that the subcutaneous route of injection offers a promising method for comparing and characterizing strains of poliovirus.
Coid et al. (1961) studied this question.