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Mathematical models of the TCA cycle derived previously for 14C tracer studies have been extended to 13C NMR to measure the 13C fractional enrichment of 2-13Cacetyl-CoA entering the cycle and the relative activities of the oxidative versus anaplerotic pathways. The analysis is based upon the steady-state enrichment of 13C into the glutamate carbons. Hearts perfused with 2-13Cacetate show low but significant activity of the anaplerotic pathways. Activation of two different anaplerotic pathways is demonstrated by addition of unlabeled propionate or pyruvate to hearts perfused with 2-13Cacetate. In each case, the amount of 2-13Cacetate being oxidized and the relative carbon flux through anaplerotic versus oxidative pathways are evaluated.
Malloy et al. (Mon,) studied this question.
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