A novel optical mapping system using spatial oversampling reliably quantified electrical activation and repolarisation in murine atria, with action potential durations comparable to standard methods.
Does a new optical mapping system with spatial oversampling accurately quantify electrical activation and repolarisation in murine atria compared to monophasic and transmembrane action potentials?
A novel optical mapping system using a 2nd generation CMOS camera with spatial oversampling provides reliable high-resolution assessment of electrical activation and repolarisation in murine atria.
We developed and validated a new optical mapping system for quantification of electrical activation and repolarisation in murine atria. The system makes use of a novel 2nd generation complementary metal-oxide-semiconductor (CMOS) camera with deliberate oversampling to allow both assessment of electrical activation with high spatial and temporal resolution (128 × 2048 pixels) and reliable assessment of atrial murine repolarisation using post-processing of signals. Optical recordings were taken from isolated, superfused and electrically stimulated murine left atria. The system reliably describes activation sequences, identifies areas of functional block, and allows quantification of conduction velocities and vectors. Furthermore, the system records murine atrial action potentials with comparable duration to both monophasic and transmembrane action potentials in murine atria.
Yu et al. (Fri,) conducted a other in Murine atria electrical activation and repolarisation. Optical mapping system using spatial oversampling (CMOS camera) vs. Monophasic and transmembrane action potentials was evaluated on Quantification of electrical activation and repolarisation. A novel optical mapping system using spatial oversampling reliably quantified electrical activation and repolarisation in murine atria, with action potential durations comparable to standard methods.