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Abstract Methodology for studies of effects of heating multilayers of human serum albumin (HSA) and anti‐HSA is presented. Multilayers of anti‐HSA were prepared on silicon substrates and studied with infrared spectroscopic ellipsometry equipped with a heat stage. The refractive index N = n + ik and the layer thickness are determined and the amide bands are analyzed. It is found that HSA/anti‐HSA multilayers are stable for shorter times at temperatures above 100 °C, except for small thickness changes. Also pilot studies of effects of heating monolayers of proteins adsorbed on gold substrates is presented. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Arwin et al. (Mon,) studied this question.