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January 1, 1998Journal of Biological ChemistryOpen Access

Selective Requirement of Myosin Light Chain 2v in Embryonic Heart Function

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Population

Myosin light chain 2v -/- mouse embryos

Comparison

Disruption of myosin light chain 2v gene vs Wild type littermates

Design

Preclinical

Follow-up

Up to embryonic day 12.5

Authors

JCJu ChenSKSteven W. KubalakSMSusumu Minamisawa

Discussion

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Overview

MLC2v is essential for embryonic cardiac contractility and morphogenesis; hypothesis-generating for human congenital cardiomyopathy mechanisms.

Key Points

  • The study aims to understand the role of myosin light chain 2v in embryonic cardiac function and its implications for cardiac development.
  • Disrupted myosin light chain 2v gene in murine models to monitor cardiac function.
  • Performed ultrastructural analysis on mutant and wild type embryos.
  • Assessed left ventricular ejection fraction to evaluate cardiac performance.
  • Mutant embryos died around embryonic day 12.5.
  • Left ventricular ejection fraction was significantly reduced in mutant embryos compared to wild type littermates.
  • Despite increased myosin light chain 2a, defects in sarcomeric assembly were observed.

Structured PICO

P
Population
Myosin light chain 2v -/- mouse embryos
I
Intervention
Disruption of myosin light chain 2v gene
C
Comparator
Wild type littermates
O
Outcome
In vivo cardiac function, survival, and sarcomeric assemblysurrogate

Myosin light chain 2v is uniquely required for maintaining cardiac contractility and ventricular chamber morphogenesis during mammalian cardiogenesis.

Cite This Study

Chen et al. (1998) studied this question.

synapsesocial.com/papers/6a7064a68031ec7bb1dc4ea6https://doi.org/10.1074/jbc.273.2.1252
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