Peroxynitrite oxidation of tetrahydrobiopterin impaired endothelium-dependent vascular relaxation in apoE-deficient mice, suggesting a pathogenic mechanism for nitric oxide synthase uncoupling.
Reactive oxygen species may alter endothelium-dependent vascular relaxation through interactions between peroxynitrite and tetrahydrobiopterin, potentially causing 'uncoupling' of NO synthase.
Background —Altered endothelial cell nitric oxide (NO · ) production in atherosclerosis may be due to a reduction of intracellular tetrahydrobiopterin, which is a critical cofactor for NO synthase (NOS). In addition, previous literature suggests that inactivation of NO · by increased vascular production superoxide (O 2 ·− ) also reduces NO · bioactivity in several disease states. We sought to determine whether these 2 seemingly disparate mechanisms were related. Methods and Results —Endothelium-dependent vasodilation was abnormal in aortas of apoE-deficient (apoE −/− ) mice, whereas vascular superoxide production (assessed by 5 μmol/L lucigenin) was markedly increased. Treatment with either liposome-entrapped superoxide dismutase or sepiapterin, a precursor to tetrahydrobiopterin, improved endothelium-dependent vasodilation in aortas from apoE −/− mice. Hydrogen peroxide had no effect on the decay of tetrahydrobiopterin, as monitored spectrophotometrically. In contrast, superoxide modestly and peroxynitrite strikingly increased the decay of tetrahydrobiopterin over 500 seconds. Luminol chemiluminescence, inhibitable by the peroxynitrite scavengers ebselen and uric acid, was markedly increased in apoE −/− aortic rings. In vessels from apoE −/− mice, uric acid improved endothelium-dependent relaxation while having no effect in vessels from control mice. Treatment of normal aortas with exogenous peroxynitrite dramatically increased vascular O 2 ·− production, seemingly from eNOS, because this effect was absent in vessels lacking endothelium, was blocked by NOS inhibition, and did not occur in vessels from mice lacking eNOS. Conclusions —Reactive oxygen species may alter endothelium-dependent vascular relaxation not only by the interaction of O 2 ·− with NO · but also through interactions between peroxynitrite and tetrahydrobiopterin. Peroxynitrite oxidation of tetrahydrobiopterin may represent a pathogenic cause of “uncoupling” of NO synthase.
Laursen et al. (Tue,) conducted a other in Endothelial dysfunction in atherosclerosis. Liposome-entrapped superoxide dismutase, sepiapterin, or uric acid vs. Untreated vessels / control mice was evaluated on Endothelium-dependent vasodilation and vascular superoxide production. Peroxynitrite oxidation of tetrahydrobiopterin impaired endothelium-dependent vascular relaxation in apoE-deficient mice, suggesting a pathogenic mechanism for nitric oxide synthase uncoupling.