Right ventricular hypertrophy induced by pulmonary artery constriction in rats reduced maximal myocyte tension at a sarcomere length of 2.0 μm (24.3 vs 37.5 mN/mm2, P<0.01).
In a rat model of right ventricular hypertrophy, depressed cardiac function is associated with decreased myocyte tension development and reduced calcium responsiveness, suggesting altered contractile protein function.
Absolute Event Rate: 24.3% vs 37.5%
p-value: p=<.01
Background The contractile dysfunction observed in end-stage myocardial hypertrophy has at its base an abnormality in myocyte function. However, whether depressed contractile function is related to an alteration in contractile protein function is presently unknown. Methods and Results Contractile force, tension, and calcium responsiveness were measured in single-skinned myocytes isolated from rats with right ventricular hypertrophy (RVH) and control rats. RVH was induced by pulmonary artery constriction for 36 weeks and was associated with significant myocyte hypertrophy. Myocytes were attached to micropipettes that extended from a force transducer and motor. Isometric force was measured over a wide range of calcium concentrations at two sarcomere lengths (SLs). Maximal force was increased in the RVH group: 1.20±0.10 versus 1.62±0.13 mg at SL=2.0 μm and 1.33±0.10 versus 1.84±0.15 mg at SL=2.3 μm ( P <.05). Maximal tension, however, was reduced in the RVH group: 24.3±1.91 versus 37.5±2.92 mN/mm 2 at SL=2.0 μm and 27.4±1.78 versus 41.8±3.19 mN/mm 2 at SL=2.3 μm ( P <.01). The concentration of calcium ions required for half-maximal activation was increased in the RVH group: 2.64±0.13 versus 3.47±0.22 μmol/L at SL=2.0 μm and 2.23±0.15 versus 2.86±0.18 μmol/L at SL=2.3 μm ( P <.01). The slope of the force-calcium relationship (Hill coefficient) was decreased in the RVH group at SL=2.0 μm (4.3±0.4 versus 3.1±0.2, P =.04) but not at SL=2.3 μm (3.8±0.2 versus 3.6±0.2, P =NS). Conclusions These results suggest that the depressed cardiac function of end-stage myocardial hypertrophy may be due, in part, to altered contractile protein function.
Fan et al. (Tue,) conducted a other in Right ventricular hypertrophy. Pulmonary artery constriction (to induce RVH) vs. Control rats was evaluated on Maximal tension at sarcomere length 2.0 μm (p=<.01). Right ventricular hypertrophy induced by pulmonary artery constriction in rats reduced maximal myocyte tension at a sarcomere length of 2.0 μm (24.3 vs 37.5 mN/mm2, P<0.01).