June 26, 2009Open Access

Respiratory viral infections detected by multiplex PCR among pediatric patients with lower respiratory tract infections seen at an urban hospital in Delhi from 2005 to 2007

Q: What is the clinical evidence from this study?

Study design: Observational. Population: Acute lower respiratory tract infections (ALRI) (n=301). Intervention: Multiplex PCR (mPCR) vs. Centrifugation enhanced culture (CEC) followed by indirect immunofluorescence (IIF). Primary outcome: Detection of respiratory viruses.

Key Result

Multiplex PCR detected 130 respiratory viruses in 35.2% of pediatric patients with lower respiratory tract infections, compared to only 79 viruses detected by conventional culture methods.

Study Design

Type

Observational (n=301)

Multicenter

Structured PICO

Does multiplex PCR improve the detection of respiratory viruses compared to conventional culture and immunofluorescence in children with acute lower respiratory tract infections?

Population

301 children ≤ 5 years of age with acute lower respiratory tract infections (ALRI) attending the outpatient department or admitted to the ward of All India Institute of Medical Sciences hospital at New Delhi, India.

Intervention

Multiplex PCR (mPCR) assay for the detection of respiratory syncytial virus (RSV), influenza viruses, parainfluenza viruses (PIV1-3) and human metapneumovirus (hMPV)

Comparator

Virus isolation by centrifugation enhanced culture (CEC) followed by indirect immunofluorescence (IIF)

Outcome

Detection rate and sensitivity/specificity of respiratory virusessurrogate

Multiplex PCR is a more sensitive method than conventional culture and immunofluorescence for detecting common respiratory viruses in children with acute lower respiratory tract infections.

Main Result

Absolute Event Rate: 130% vs 79%

Limitations

Did not make direct comparisons of the performance of the different multiplex PCR assays in the laboratory
The number of all viral detections except RSV was too few to comment on the association of the virus with bronchiolitis or pneumonia
CEC-IIF could not detect the presence of viruses in samples with mixed infections

Abstract

BACKGROUND: Acute lower respiratory tract infections (ALRI) are the major cause of morbidity and mortality in young children worldwide. Information on viral etiology in ALRI from India is limited. The aim of the present study was to develop a simple, sensitive, specific and cost effective multiplex PCR (mPCR) assay without post PCR hybridization or nested PCR steps for the detection of respiratory syncytial virus (RSV), influenza viruses, parainfluenza viruses (PIV1-3) and human metapneumovirus (hMPV). Nasopharyngeal aspirates (NPAs) were collected from children with ALRI 0.05). CONCLUSION: Multiplex PCR offers a rapid, sensitive and reasonably priced diagnostic method for common respiratory viruses.

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Cite This Study

Bharaj et al. (Fri,) conducted a observational in Acute lower respiratory tract infections (ALRI) (n=301). Multiplex PCR (mPCR) vs. Centrifugation enhanced culture (CEC) followed by indirect immunofluorescence (IIF) was evaluated on Detection of respiratory viruses. Multiplex PCR detected 130 respiratory viruses in 35.2% of pediatric patients with lower respiratory tract infections, compared to only 79 viruses detected by conventional culture methods.

synapsesocial.com/papers/6a08858c113ba5b476de3dd9 https://doi.org/https://doi.org/10.1186/1743-422x-6-89

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