A new micromethod for measuring renin activity in 0.1 ml of rat plasma and 1 mg of renal tissue demonstrated excellent reproducibility with 77% recovery of angiotensin.
A new micromethod for the measurement of renin activity and substrate in 0.1 ml of rat plasma and in 1 mg of rat renal tissue is described. In the procedure a rat substrate free of renin prepared from previously nephrectomized animals is used. Incubation is carried out in the presence of Dowex resin 50W-X2 (NH 4 + ) for 12 hours, at pH 6.5, with an excess of rat substrate. Angiotensin formed during incubation is measured by a pressor assay in the rat. The reproducibility of the procedure was excellent. Recovery of angiotensin in amounts from 10 to 500 mμg was 77%. This method permits repeated measurements of renin activity in rats during acute experiments and in any physiological procedure. It can readily be adapted to other species.
Boucher et al. (Fri,) reported a other. Micromethod for measurement of renin was evaluated on Recovery of angiotensin. A new micromethod for measuring renin activity in 0.1 ml of rat plasma and 1 mg of renal tissue demonstrated excellent reproducibility with 77% recovery of angiotensin.