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Enzyme hydrolysis of zein−wax-coated paper was investigated as part of an effort to develop a recyclable waxed paper product. Brown Kraft paper was coated with corn zein and paraffin wax, and the grease proofing and water vapor barrier properties of the paper were determined. Grease permeation was found to depend on the amount and the method of application of zein present in the zein−wax coating. Water vapor transmission values were strongly dependent on the amount of wax in the coating. We have shown that α-chymotrypsin was able to hydrolyze the zein and cleanly separate the coating from the paper fibers. Treatment with Tris buffer pH 8.5, containing 0.1% SDS and α-chymotrypsin (E:S ratio = 1:100) for 0.5 h effectively removed more than 97% of the zein−wax coating from the paper. Zein removal was followed by SDS−capillary electrophoresis of liquid samples taken during hydrolysis. SEM images of the extraccted paper indicated that the paper fibers were free of coating material. Keywords: Zein; paraffin wax; coating; paper; recycle; enzyme; hydrolysis; digestion; α-chymotrypsin
Parris et al. (Thu,) studied this question.