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Flat preparations of the retina afford an unusually favorable opportunity to study blood vessel architecture (Ballantyne and Lowenstein 1 ). The retinal vessels have been visualized in the past by the following methods: 1. injection of India ink into the heart, major arteries, or directly into the retinal artery (Michaelsen, 2 Ashton, 3 Keeney and Barlow, 4 and others); 2. latex injection into the aorta (Janes 5 ); 3. benzidine-peroxidase staining of the red blood cells (Michaelsen 2 ); 4. infusion of silver nitrate with subsequent reduction of the silver to outline the reticular fibers, 6 and 5. staining of the full thickness of
Toichiro Kuwabara (Thu,) studied this question.