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SUMMARY: Nitrosomonas europaea (Winogradsky) was isolated in pure culture from Rothamsted soil. The method used entailed: (1) building up the population of nitrifiers in enrichment cultures; (2) removal of the cells from the chalk in an enriched culture with a stream of carbon dioxide; (3) picking colonies from poured silica gel plates.
Jane Meiklejohn (Mon,) studied this question.
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